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SM ISO690:2012 DESYATNIK, A., TIURINA, T., GUTSUL, Tatiana, CLAPCO, Steliana, BIVOL, Cezara, NICORICI, Alexandr, RUSU, Emil, LABLYUK, S., DVORNINA, Elena. Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes. In: NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies, Ed. 2016, 11-14 mai 2016, Chişinău. Chișinău, Republica Moldova: 2016, p. 10. |
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NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies 2016 | ||||||
Conferința "NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies" 2016, Chişinău, Moldova, 11-14 mai 2016 | ||||||
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To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic |
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<?xml version='1.0' encoding='utf-8'?> <oai_dc:dc xmlns:dc='http://purl.org/dc/elements/1.1/' xmlns:oai_dc='http://www.openarchives.org/OAI/2.0/oai_dc/' xmlns:xsi='http://www.w3.org/2001/XMLSchema-instance' xsi:schemaLocation='http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd'> <dc:creator>Ciloci (Deseatnic), A.A.</dc:creator> <dc:creator>Tiurina, T.</dc:creator> <dc:creator>Guţul, T.D.</dc:creator> <dc:creator>Clapco, S.F.</dc:creator> <dc:creator>Bivol, C.M.</dc:creator> <dc:creator>Nicorici, A.</dc:creator> <dc:creator>Rusu, E.V.</dc:creator> <dc:creator>Labliuc, S.V.</dc:creator> <dc:creator>Dvornina, E.G.</dc:creator> <dc:date>2016</dc:date> <dc:description xml:lang='en'><p>To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic<br />enzymes metal oxide nanoparticles: MgO, ZnO, ZnO/MgO in a ratio of 1:4, TiO2 and Fe3O4<br />through hydrothermal and sol-gel techniques were synthesized. These nanomaterials were<br />characterized by powder X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR)<br />and SEM-microscopy.<br />The assessment of the influence of synthesized nano metal oxides, was carried out<br />according to their impact on biosynthesis of the enzyme at micromycetes Trichoderma<br />koningii, Fusarium gibbosum and Aspergillus niger – producers of extracellular protease and<br />amylase.<br />It was found that the impact of nanomaterials on the synthesis of hydrolases depends<br />on the structure, dimension and concentration of the nanoparticles used as well as on the<br />physiological and biochemical characteristics of the strain and the specificity of action of the<br />enzymes synthesized by them. According to the results of research, ZnO, ZnO/MgO and<br />Fe3O4 nanoparticles may be considered as stimulators of biosynthesis of extracellular<br />proteases in micromycetes Trichoderma koningii and Fusarium gibbosum. However, the<br />degree of their exposure to proteolytic complexes synthesized by two strains was different. In<br />Trichoderma koningii strain this compounds increased the enzyme activity of both acid (by<br />12.1-22.3%) and neutral proteases (by 121.9-188.1%). In Fusarium gibbosum strain the<br />stimulating effect caused by the action of nanoparticles was less significant (by 30.5-40.0%)<br />and occurred only for neutral proteases. For the biosynthesis of amylase synthesized by<br />micromycete Aspergillus niger, the studied a group of nanoparticles had mostly inhibitory<br />effect (by 14.9-54.8%).<br />Biotechnological interest present ZnO nanoparticles of 30nm, which in the<br />concentration of 5-10mg/L in the experimental conditions increased the activity of neutral<br />proteases, as the main component of the enzyme complex strains, up to 3 times.</p></dc:description> <dc:source>NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies () 10-10</dc:source> <dc:title>Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes</dc:title> <dc:type>info:eu-repo/semantics/article</dc:type> </oai_dc:dc>