Microorganisms are the main source of various bioactive substances used in diverse fields of biotechnology. A current problem in agriculture is the fight against various phytopathogens, which cause crop diseases. Current trends worldwide are green agricultural methods. Actinobacteria, bacteria, and micromycetes are known to naturally associate with plants and have a beneficial effect on their growth. Thus, the groups of microorganisms listed above were isolated from the silt samples of the "La izvor" lake system (Chisinau municipality), and their screening was performed to combat phytopathogenic agents that cause plant diseases. The following strains of microorganism actively inhibit the growth of phytopathogenic fungi: A. alternata - 8 micromycetes, 2 bacteria; A. niger - 1 micromycete, 3 actinobacteria; B. cinerea 5 micromycetes, 4 bacteria; F. oxysporum - 7 micromycetes, 3 actinobacteria, 2 bacteria. The growth of phytopathogenic bacteria was actively inhibited only by micromycetes: B. subtilis - 15 strains; X. campestris - 8 strains; C. michiganensis - 5 strains; A. tumefaciens - 3 strains; E. carotovora - 5 strains.