The purpose of the research is to evaluate the haploproduction capacity of the breeding material of bread wheat and to create high-protein lines by androgenesis in vitro. One of the urgent tasks of biotechnology today is the homozygization of genetically unstable breeding material with specific marker traits, which is the result of the destruction of wheat with introgressive lines. The research material was 13 genotypes provided by the wheat breeding department of the SGI-NCNS: varieties Kuyalnyk, Tradition, introgressive lines 9300, 9525 and 3238 (the latter obtained as a result of distant crossings of cultivated wheat with Aegilops tauschii (line 3238;) and Triticum dicoccoidles (line 9225; 9300) with subsequent selection and saturation crossings) and 8 hybrids of the first generation between these genotypes Microspores of all genotypes formed calluses in anther culture in vitro were showed. The intensity of the last process was low (from 0.47 to 3.0%). The regenerative capacity of the new formations did not exceed 0.34% of the planted anthers. Green regenerates in anther culture of ten genotypes have been received. A total of 24 green regenerants were obtained by in vitro androgenesis, from which, after the stages of adaptation to soil conditions, vernalization and growth, seeds of seven dihaploid lines were obtained. Two lines each in the anther culture of the hybrid F1 Tradition / line 9300 and the Kuyalnik variety and one of the combinations of crosses F1 Line 9300 / Line 3238, F1 Line 9300 / Tradition and line 9525. As a result of the study, it was shown that the effectiveness of the anther culture method for obtaining high-protein linear material of soft winter wheat depends on the donor material. In our research, we did not use diploidizers, that is, we obtained all 7 fertile plants - this is the result of spontaneous diploidization. The value of spontaneous diploidization ranged from 0 to 100% depending on the genotype, with an average of 29.17%, which correlates with similar results of another researches. Thus, the low haploproduction potential in in vitro anther culture of genotypes carrying in the genome foreign genetic material with the GPC-B1 gene from Ae. tauschii and T. dicoccoidles have been showed. However, 30% of the obtained regenerative plants are dihaploid lines that are the result of spontaneous diploidization.