We studied the biochemical composition and nutritive value of the green mass and prepared hay from Ucrainan and Romanian cultivars of bird’s-foot trefoil Lotus corniculatus „Aiaks”, „Dacia 1”, „Doru”, „Măgurele 8” grown in monoculture on the experimental plot of the National Botanical Garden (Institute) “Alexandru Ciubotaru”, Chisinau, R. Moldova. Results revealed that dry matter of harvested green mass contained 179-189 g/kg CP, 233-294 g/kg CF, 100-102 g/kg ash, 261-325 g/kg ADF, 420-514 g/kg NDF, 40-51 g/kg ADL, 221-254 g/kg Cel, 159-189 g/kg HC, 97-182 g/kg TSS, with nutritive and energy value 70.9-78.5% DMD, 60.0-72.0% ODM, RFV=115-172, 10.26-11.16 MJ/kg ME and 6.29-7.02 MJ/kg NEl. The biochemical composition, nutritive and energy value of prepared hay from cv. Aiaks and cv. Dacia 1: 201-206 g/kg CP, 284-314 g/kg CF, 108-109 g/kg ash, 303-335 g/kg ADF, 455-517 g/kg NDF, 40-43 g/kg ADL, 263-293g/kg Cel, 152-179 g/kg HC, 45-65 g/kg TSS, RFV=113-134, 10.12-10.53 MJ/kg ME and 6.14-6.54 MJ/kg NEl. The studied cultivars of bird’s-foot trefoil Lotus corniculatus could be used in the Republic of Moldova for the restoration of degraded permanent grasslands, as a component of the mix of grasses and legumes for the creation of temporary grasslands, also can be planted between rows in vineyards and orchards, the harvested biomass can be used as feed for animals.