QPCR detection and quantification of &lsquo;Candidatus phytoplasma solani&rsquo; in tomato with primers targeting CPN60 gene

Mitina Irina; Bahşiev Aighiuni; Mitin Valentin; Zamorzaeva-Orleanscaia Irina

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2024-03-21 14:07

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632.9:635.64 (1)

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MITINA, Irina, BAHŞIEV, Aighiuni, MITIN, Valentin, ZAMORZAEVA-ORLEANSCAIA, Irina. QPCR detection and quantification of ‘Candidatus phytoplasma solani’ in tomato with primers targeting CPN60 gene. In: Genetica, fiziologia şi ameliorarea plantelor, Ed. 7 , 4-5 octombrie 2021, Chişinău. Chişinău: "Print-Caro" SRL, 2021, Ediția 7, pp. 79-82. ISBN 978-9975-56-912-5. DOI: https://doi.org/10.53040/gppb7.2021.20

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Genetica, fiziologia şi ameliorarea plantelor
Ediția 7, 2021

Conferința "Genetica, fiziologia şi ameliorarea plantelor"
7 , Chişinău, Moldova, 4-5 octombrie 2021

QPCR detection and quantification of ‘Candidatus phytoplasma solani’ in tomato with primers targeting CPN60 gene

DOI:https://doi.org/10.53040/gppb7.2021.20

CZU: 632.9:635.64

Pag. 79-82

Mitina Irina, Bahşiev Aighiuni, Mitin Valentin, Zamorzaeva-Orleanscaia Irina

Institute of Genetics, Physiology and Plant Protection

Proiecte:

Menționat în lucrare: 20.80009.5107.11 Conservarea ex-situ de lungă durată a resurselor genetice vegetale în banca de gene cu utilizarea metodelor biologiei moleculare în testarea stării de sănătate a dermoplasmei vegetale

Menționat în lucrare: 18.80013.5107.05. STCU/6378 Elaborarea tehnicii noi de evaluare a rezistenței tomatelor la fitroplasmă

Disponibil în IBN: 4 octombrie 2021

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Rezumat

Tomato is one of the most economically important crops in Republic of Moldova. However, it is affected by a number of pathogens. One of the wide spread diseases is stolbur caused by the infection agent ‘Candidatus Phytoplasma solani’. Accurate diagnostics of the disease at an early stage is essential for successful control of the disease. In this work, we describe detection and quantification of ‘Candida-tus Phytoplasma solani’ in tomato by real-time PCR, as well as suitability of the method for assessing resistance of different tomato varieties to Phytoplasma infection.

Cuvinte-cheie
‘Candidatus phytoplasma solani’, stolbur, tomato, Real-Time PCR

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