One of the main methods of agrobiodiversity preservation is the ex situ conservation of cultural plants germplasm. Among the numerous problems arising during seed material conservation the seed aging is ones of the important because it directly affects the obtaining of genetic homogenous healthy generation. For the increasing of the seeds viability after their conservation different methods are used, including the influence of the biological active substances. The purpose of the investigation was the research of the influence of some natural bioregulators on the old wheat seeds. The influence of the steroidal glycosides Sisymbrifoliozid and Ecostim on the germination of the collection samples of wheat after their long conservation in the Gene Bank of plants of Moldova were studied. The seeds of more than 50 wheat genotypes (reproduction 1992) were tested. The seeds were soaked for 18 hours in water solutions of bioregulators in a concentration from 10-1 up to 10-4 and then registered according to ISTA techniques. The variety-specific reaction of the wheat on the aging process and the application of the concrete steroidal glycoside were revealed. The action of different concentrations of bioregulators also manifested differentially on these seeds. After the treatment of the old seeds with 0,01% solution the Ecostim energy of the germination and germination increased approximately 1,3 – 1,5 times at the majority of the genotypes of the wheat in comparison with the control (distilled water), and the seeds had more developed and viable rootlets. The maximal stimulating effect on the processes of germination rendered the treatment of the seeds by Sisymbrifoliozid. So, for example, at such genotypes as Tavricheanka, Stepneak and Birlik the energy of the germination and germination increased 1,5-1,8 times in comparison with the control. Thus, the stimulating action of the natural bioregulators of a steroidal nature Sisymbrifoliozid and Ecostim on the processes of the germination of winter wheat genotypes it was observed and their optimal concentration were revealed. The received results permit to recommend these chemical preparations for increasing the viability of the wheat seeds during the ex situ conservation.