The genus Rhodococcus comprises Gram-positive actinobacteria that are widely distributed in the environment, including in soil and water from tropical, desert and artic habitats. Rhodococcus have a high destructive potential against a wide range of aromatic, heterocyclic and aliphatic xenobiotics, and are also producers of valuable secondary metabolites (surfactants, exopolysaccharides, enzymes) [1]. The strain Rhodococcus wratislaviensis G13 was isolated from oil sludge and added to the collection of microbial xenobiotic decomposers of the Laboratory of Environmental Biotechnology of the Institute of Microbiology, NAS of Belarus. In this study, we carried out an initial screening of the strain R. wratislaviensis G13 and studied its substrate specificity towards a wide range of aliphatic and aromatic organic compounds. The bacteria R. wratislawiensis G13 was maintained on Meat-wort agar (5% meat-peptone broth, 25% filtered wort, 2,2% agar, pH 7,0) at 25-27°C for 48-72 h. Screening for destructive bacterial activity of R. wratislaviensis strain G13 was carried on M9 medium (Na2HPO4, 33.7 mM; KH2PO4, 22.0 mM; NaCl, 8.55 mM; NH4Cl, 9.35 mM; MgSO4, 1.0 mM; CaCl2, 0.3 mM) [2] containing individual chemical compounds as the main source of carbon at a concentrations of 0.02%, 1% and 5%. The G13 strain was also inoculated on M9 control medium without pollutants, in order to exclude agarolytic cultures. The destructive activity of the strain was assessed after 7 days. According to the results of the initial screening, we have shown the potential biodegradability of aliphatic organic compounds (propanol, butanol, acetone, ethyl acetate, butyl acetate at a concentration of 5%), benzene 5%, phenol 0.02%, oxyhydroquinone and catechol 1%, naphthalene and their derivatives (α-naphthol, naphthylamine, α-naphthyl acetate at a concentration of 1%) as well as complex organic solvents (solvent and Stoddard solvent at a concentration of 5%) by R. wratislaviensis G13. In addition, this strain is an active producer of surfactants and exopolysaccharides, allowing it to withstand high concentrations of toxicants under various extreme environmental conditions. All this made it possible to include G13 strain into the «Rhodobel-TN» biosorption preparation intended for soil and water treatment from oil and products of its processing. It is worth noting that the range of activity conditions of this preparation is wide enough: pH 4-10 and temperature 345°C. The strain has high xerotolerance and heat resistance due to the production of exopolysaccharides, which have a protective function when the microbial cells are dried. This fact together with the powerful destructive potential of the strain to a wide range of xenobiotics became the basis for inclusion of R. wratislaviensis G13 in the dry preparative form of bioactivator «Antoil+C» intended for municipal wastewater treatment. Thus, a further detailed analysis of the characteristics of the R. wratislaviensis G13 strain will make it possible to substantiate in more detail its potential as a biodegradable agent in microbial preparations.