The biologically active compounds are present in biological samples in relatively low concentration levels. Hence, an essential step is isolation from complex matrices (whole blood, tissue) and enrich them before the final determination. From a medical point of view, the routine analysis of xenoestrogens, which are one of the causes of cancer, generates a lot of problems. Interest of the analytics of this group of compounds need to be developed effective analytical approaches for biological monitoring of xenoestrogens and their derivatives. Solid phase extraction with new generation sorbents and their miniaturized generation fibers are alternative for the implementation of determination by conjugated and multidimensional separation techniques. To enlarge specificity/selectivity, sorbents will be modified with appropriate chosen molecules, which will create a molecular recognition system or even monolithic columns for SPE coupled on-line with micro- and/or nanocolumns for electrochromatography, capillary electrophoresis or liquid chromatography. Fundamental research realized within the presentation, is focused on two main areas, which include macromolecular and supramolecular chemistry and research of the relationship between the structure and properties of polymers and biotechnology combined with enzymatic applied microbiology. Using the ideas of the superconducting triplet spin-valve and developed advanced vacuum technology, we have fabricated layered functional nanostructures Nb/Co/CuNi , were triplet pairing with switching from normal to superconducting state and a memory effect has been detected. The designed S/F functional nanostructures can serve as the rapid operating switching element of superconducting electronics and as the memory element MRAM for novel computers generation.