Selective breeding of frost-resistant grape varieties is a compelling and challenging task. One of the critical stages in this work is the selection of initial varieties that will become donors of valuable traits. The most complete and reliable data on frost resistance of grape varieties can be obtained only as a result of field and laboratory tests. The purpose of the study was to determine frost resistance level of new table and wine grape varieties and hybrids using laboratory freezing method, and select the most promising varieties and hybrids for inclusion into the selection process. The study analyzed 16 varieties and 28 grape hybrids. According to the data obtained in the course of the study, the highest frost-resistance among the seedless varieties was demonstrated by Kishmish Aksaysky grapes. More than 40% of the cuttings developed into plants after having been frozen at minus 25 ° C. Among table varieties and hybrids, the highest level of frost resistance was observed in Muscat of Crimea variety. At minus 24 °С, 40% of the eyes were preserved, at minus 25 °С - 14.3% were preserved. In the group of wine varieties, Alminsky grapes demonstrated high frost resistance with complete eye preservation at minus 26°C. Magarach № 8-08-8-4 (Kok Pandas x Zeybel 6357) became a promising hybrid in this group. At a temperature of minus 27 °C, shoot sprouting constituted 66.7% from secondary buds; at minus 26 °C - 50% from the main buds. As frost resistance sources, hybrids Magarach № 8-10-20-2 (minus 26 °C), Magarach № 530 (minus 27 °C) and Magarach № 512-243-1 (minus 27 °C) were singled out. The study was conducted within the framework of public assignment № 0833-2015-0015.