It has been observed that human and animal intestinal microbiota in individuals with normal physiological state consists of rod-shaped (bacilli) and spherical (cocci) bacteria. Analysis of existing bibliographic sources has revealed that the elaboration of bio probiotics for reconstituting the normal bacterial flora in case of microbial imbalance is realized preponderantly using rod-shaped bacteria and only very limited instances when cocci are used (only representatives of the Enterococcus genus). Thus the objective of this paper is to study the intestinal cocci of healthy gut microbiota in human and animal individuals of various ages, highlighting those specific of the digestive tract and the opportunity to include them in new probiotic bioproducts. This objective has been achieved as a result of scientific research carried out in several stages. The first - was dedicated to the isolation of streptococci from healthy intestinal content; second - their identification and third - calculating the percentage of the various genera of specific intestinal streptococci in children and young farm animals of different age. As a result, 1108 strains of streptococci were isolated from the intestinal content. Among them, 834 strains specific to 1–16 years old children, 154 to swine and 120 to bovine, this constituted respectively 75, 27%, 13, 89% and 10.83% of the total number of isolates. After identification, these were assigned to three genera: genus Lactococcus, the most present in 1–3 year children, ranging from 46.31 to 46.98%; genus Streptococcus was the second, with 31,57 to 37.73%, and the third – Enterococcus, with18,66 to 22.10%. It is important that for 5–16 years old children was observed quantitative preponderance of Enterococcus (38.18% - 46.77%), decrease in the number of (from 28.18% to 13.70%) and maintaining the proportion of the genus Streptococcus (33.92 to 39.51%). As the streptococci isolated from children were present in a larger quantity, some of their basic probiotic properties were determined (the antagonistic activity and the adhesion ability). Thus 301 Enterococcus strains expressed anti-Escherichia activity (85,45% to 89,35%), anti- Proteus activity (71,25% to 77,57%) and anti-Staphylococcus activity (78,40% to 85,70%). For 297 strains of Streptococcus this activity was lower, ranging from 72,35% to 79,69%; 69,64% to 75,40%; 74,55% to 82.60% respectively. Finally, 236 strains of Lactococcus expressed the lowest antagonistic activity, respectively from 68,75% to 70,37%; from 59,45% to 65,60% and 71,25% to 76,75%. The adhesion index was as well the highest for the Enterococcus bacteria (4,58 – 4,77), followed by Streptococcus (3,66 – 4,37) and Lactococcus - ranging between 3,16 and 3,70 c.u. Therefore, it was determined a high incidence of representatives of Streptococcaceae family in healthy intestinal microflora of children and young farm animals of different age. Their quantitative and qualitative composition, as well as their utility for organism differs depending of studied groups. Therefore, is recommended to use such microorganisms for the elaboration of new bio probiotic products, and their composition for children must be differentiated by age groups (Genus Lactococcus 1–3 years; Enterococcus 5–16 years and Streptococcus1–16 years).