Creosote is a wood-preserving product obtained by fractional distillation of crude coal tar produced by high-temperature carbonization of bituminous coal. It is composed of approximately 85% polycyclic aromatic hydrocarbons (PAH), 10% phenolic compounds, and 5% N-, S-, and O-heterocyclics. Many species of creosote components are listed on the top 100 toxic substances priority chart published by the Agency for Toxic Substances and Disease Registry The aim of study was to assess effect of creosote on activity of soil phosphatases: acid and alkaline phosphomonoesterases, phosphodiesterase, phosphotriesterase and inorganic pyrophosphatase. The experiment was carried out in soil material collected from the topsoil of Brunic Arenosol in Agricultural Experimental Station in Lipnik (53°24'N, 14°28'E), located in the West Pomeranian District, Poland. Different dosages of creosote: 0, 2, 10 and 50 g·kg-1, were applied to soil samples. The soil samples were adjusted to 60% maximum water holding capacity, and they were incubated in tightly closed glass containers at a temperature of 20°C. Activity of soil enzymes was determined on days: 1, 7, 14, 28, 56 and 112. Obtained results showed that soil contamination with creosote caused decrease in activity of soil phosphatases, which increase, together with increase in creosote dosages. Among measured soil enzymes, inorganic pyrophosphatase was the most vulnerable to creosote contamination.