By the method of confocal nonlinear optical microscopy, microstructures of β- and γ-glycine were investigated. These polymorphs of glycine are strongly non-centrosymmetric, so it can be used as a biocompatible nonlinear optical and piezoelectric material [1]. β-glycine has been long recognized as a potential nonlinear optical material in which the useful optical properties (large nonlinear optical coefficient, large birefringence, wide transparency range, broad spectral and temperature bandwidth, etc.) are combined with the ability of chemical modification using molecular engineering and intrinsic biocompatibility [2,3]. The most useful β-polymorph is much less stable that the other polymorphs of glycine, but in this work we used stable microcrystals of β-glycine using (111)/Pt/SiO2/Si substrate as a template. Second harmonic generation images of the samples were measured. Also polarization and azimuthal dependences of second harmonic from certain points of the samples were measured. It was found, that values of nonlinear optical susceptibility of glycine microcrystals are equal in order of magnitude of nonlinear susceptibility of quartz (~1 pm/V). On the basis of theoretical modeling and approximation of the experimental results the components of nonlinear susceptibility tensor were calculated.