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USATÎI, Agafia; BEŞLIU, Alina; EFREMOVA, Nadejda; CHISELIŢA, Natalia; BATÎR, Ludmila; DADU, Constantin; TĂNASE, Ana. Biosynthesis of polysaccharides in Rhodosporidium Toruloides, cultivated in the presence of Fe3O4 (50–100 NM) nanoparticles. In: Microbial Biotechnology. Ediția 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, p. 155. ISBN 978-9975-3178-8-7.
Ediția 4, 2018
Conferința "Microbial Biotechnology" |
Chișinău, Moldova, 11-12 octombrie 2018
Microbial polysaccharides are located in the cell membrane; the polymerization degree and chemical structure are individual characteristics of each strain. The functionality of yeasts polysaccharides, mostly composed of β-glucans, mannoproteins, chitin depends on the cultivation conditions. From a biotechnological point of view, the efficiency of polysaccharides production can be increased by stimulating the biosynthesis process or by achieving greater productivity of cellular biomass. In this paper are presented the results of Fe3O4 (50-100 nm) nanoparticles in concentrations from 0.5 mg/L to 30 mg/L on the cell biomass and polysaccharides production in Rhodosporidium toruloides CNMN-Y-30. Fe3O4 nanoparticles (SIGMA-ALDRICH product) in the form of powder, surface area> 60 m2/g, density 4.8-5.1 g/ml at 250C refer to the category of biomedical materials Analysis of data on the influence of Fe3O4 (50-100 nm) nanoparticles on cell biomass production revealed that after 120 hours of contact, the yeast reacted positively to the concentrations applied in the experiments. According to the studies, nanoparticles at concentrations of 0.5 - 30 mg/L exhibit stimulatory effect on cell biomass production, the highest 24% increase compared to the control was recorded for the 20 mg/L concentration. The nanoparticles influence on polysaccharide biosynthesis processes is evident, depending on the concentrations applied in the nutritive medium. The obtained results demonstrate that the polysaccharides amount in yeast biomass at cultivation of strain in the presence of 0.5- 5 mg/L nanoparticles practically has not changed compared to the control samples. Administration of higher concentrations of nanoparticles substantially reduced the polysaccharides production. Research has revealed that nanoparticles used in the culture medium at concentrations of 25 and 30 mg/L reduced the polysaccharide content in yeast biomass by 35.2...37.2% compared to the control. To determine the role of nanoparticles concentrations in the process of polysaccharides accumulation, correlation analysis was performed. The correlation between the amount of polysaccharides and nanoparticles concentrations used for the cultivation of Rhodosporidium toruloides CNMN-Y-30 determined a strong dependence between the values of these parameters, (R2 = 0.8359), therefore in 83 cases the concentration of nanoparticles determines the accumulation of polysaccharides in the microbial cell. In conclusion of the study, Fe3O4 nanoparticles with 50-100 nm sizes in concentrations from 0.5 to 30 mg/L affected biochemical systems of polysaccharide synthesis and their effect depends on the used concentration. In perspective, these results may contribute to the development of theoretical and practical bases in the context of studies on the use of Fe3O4 nanoparticles in nanobiotechnology.