The development and use of biological preparations based on plant secondary metabolites is an important trend in modern organic farming. The aim of this study was to determine the biological activity of extracts and essential oils from some species of Coniferous plants, namely Juniperus sabina L. and Pinus sylvestris L., growing in Republic of Moldova (RM) and in Slovak Republic – Nitra (SN) and Pianiny mountains (PM). The antioxidant activity of ethanolic extracts of Coniferous was determined by two procedures with appreciation of their radical scavenging capacities against DPPH and peroxyl free radicals. The trolox and gallic acid serve as standard antioxidant substances. The antioxidant activity of plant extracts was evaluated in equivalent of both standard substances and by indexes IC50 (the extract concentration that scavenged 50 % of free radicals). According to all determined indexes the antioxidant activity of tested extracts from Coniferous plants was qualified in followed order: J. sabina SN ˃ J. sabina PM > J. sabina RM ˃ P. sylvestris RM ≥ P. sylvestris SN. It should be mention that the scavenging of the same amount of peroxyl radicals was carried out with the lower concentrations of alcoholic extracts than to inhibit of DPPH radicals. Therefore, the indexes IC50 (mg per g of dry residue) of extracts against DPPH and peroxyl free radicals were equal, respectively: 1.64±0.01 and 0.13±0.01 for J. sabina SN; 1.93±0.01 and 0.15±0.04 for J. sabina PM; 2.60±0.17 and 0.16±0.02 for J. sabina RM; 6.68±0.64 and 0.41±0.03 for P. sylvestris RM; 7.28±0.55 and 0.51±0.04 for P. sylvestris SN. Moreover, the biopesticidal (insecticidal, ovicidal, antifeedant, repellent and deterrent) properties of Coniferous plant extracts and oils were studied. As a result of biological activity testing it was established that both ethanolic extracts and essential oil J. sabina possessed high efficiency against Colorado Potato beetle Leptinotarsa decemlineata Say and wax moth Galleria mellonella L. Mortality of imago and larvae consisted in average 6.7–53.3 and 66.7– 100 % respectively, ovicidal activity was 100 %, and antifeedant effect persisted at the level of 1–3 points for imago and 1 point for larvae. It was established that the treatment of feed and insects with the essential oils of P. sylvestris needles resulted in the death of only 6.7–13.3 % of the wax moth larvae. There was also noted a decrease at the rate of formed pupae and emergence imagoes by 20 % compared with the control. It was determined that essential oils had a deterrent effect on egg-laying of the wax moth. Extracts from J. sabina exhibited a selective effect on various groups of insects and, therefore, allow preserving the biodiversity of agrocoenosis, which is favorable for entomophagous. The results will serve as a basis for the selection of the most effective plant extracts for further their use to protect plants from pests, as well as to increase the resistance of plants to diseases and other adverse environmental factors.