Background: Antibiotic resistance is recognized as a major problem of the medical system. Excessive antibiotic therapy reduces bacterial biodiversity by favoring colonization of the human organism with germs resistant to various antimicrobials. Material and methods: Retrospective analysis of bacteriological examinations during January 1, 2016 – December 31, 2017, collected in the ICU, Municipal Clinical Hospital Nr. 1, Chisinau. The criteria for the European Center for Disease 2012 were used to define multidrug resistance. Results: 530 analyses were investigated and were obtained 497 cases of bacterial strains. The following MDR strains were identified: a) P. aeruginosa strains showed resistance levels to: Piperacillin-tazobactam (2016 – 13%, 2017 – 100%); Levofloxacin (2016 – 53%, 2017 – 55%); Tobramycin (2016 – 33%, 2017 – 59%); Meropenem (2016 – 100%, 2017 – 69%). b) S. aureus strains showed resistance levels to: Doxycycline (2016 – 0%, 2017 – 100%); Erythromycin (2016 – 39%, 2017 – 50%); Clindamycin (2016 – 31%, 2017 – 43%). c) Enterococcus spp. showed resistance levels to: Meropenem (2016 – 100%, 2017 – 100%); Gentamicin (2016 – 80%, 2017 – 75%); Doxycycline (2016 – 67%, 2017 – 62%). d) Enterobacteriacae showed levels of resistance to: Cefuroxime (2016 – 100%, 2017 – 100%); Cefepime (2016 – 67%, 2017 – 90%); Gentamicin (2016 – 45%, 2017 – 62%); Meropenem (2016 – 100%, 2017 – 73%). Conclusions: 1) Isolated strains recorded high levels of resistance to aminoglycosides, cephalosporins and tetracyclines. 2) Analysis of MDR patterns in the isolates showed high resistance levels for all strains. 3) Strains with extended resistance (XDR) and pan-resistance (RDP) were not identified according to the criteria of the European Center for Disease.