The propose of study had served 21 strains of micromycetes from the National Collection of Non-pathogenic Microorganisms from Institute of Microbiology, Academy of science of Moldova and two pathogenic strains – Aspergillus flavus and Aspergillus niger. The tested strains were isolated from soil samples taken from the central zone of Moldova. As nutrient medium for isolation, of micromycetes served malt agar, Czapek, Sabouraud-Amidono and ammonium. Isolation of strains was performed in thermostat at temperature 28oC, during 14 days. As a result of screening were selected two strains SP.62 and SP. 97 with potential antifungal increased against Aspergillus flavus pathogen with higher antifungal actions against petrified offspring of bees. The diameter of inhibition zone of Aspergillus flavus was on the strain SP. 62 - 30 mm, and of the strain p. SP. 97 - 35 mm.