Introduction. Urinary tract infection (UTI) is one of the most common infections encountered in clinical practice, and microbial resistance is currently one of the most serious threats to global health. Objective of the study. To identify the bacterial spectrum causing UTIs in the urology department of the «Timofei Moșneaga» Clinical Hospital in 2022 and its antibiotic resistance. Material and methods. Retrospective study of urine culture results, collected in the urology department of the «Timofei Moșneaga» Clinical Hospital during 2022, samples were examined using VITEK 2 Compact (bioMérieux). Results. Total: 900 samples, sterile - 597 (66.33%), 303 (33.67%) showed bacterial growth (>105), of which E. coli - 76 (28%) cultures, sensitive to amikacin in 88.16% cases, to nitrofurantoin in 86.84% cases, to amoxicillin/clavulanate in 69.74% cases, and pan-resistant in 1.31% of cases; Klebsiella spp. - 66 (25%) cultures, sensitive to amikacin in 62.12% cases, to meropenem in 40.9% cases, to amoxicillin/clavulanate in 39.39% cases, and panresistant in 12.12% of cases; Enterococcus faecalis - 53 (20%) cultures, sensitive to ampicillin in 71.7% cases, to amoxicillin/clavulanate in 67.92% cases, to piperacillin/tazobactam in 62.26% cultures; Pseudomonas aeruginosa - 21 (8%), sensitive to amikacin in 47.62% cases, to meropenem in 47.62% cases, to piperacillin/tazobactam in 19.05% cases, and pan-resistant in 14.29% of cases; the remaining 87 (19%) cultures were other species. Conclusions. Urine cultures that were examined from the patients hospitalized in Urology Department in 2022, was determed: a higher incidence of Klebsiella spp. and Enterococcus faecalis, high sensitivity to aminoglycosides and aminopenicillins.