Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
66 0 |
SM ISO690:2012 IVĂNESCU, Larisa Maria, ACATRINEI, Dumitru Mihai, PAVEL, Ionuţ, ŞULEŞCO, Tatiana, MIRON, Liviu Dan. PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania. In: Acta Parasitologica, 2015, vol. 60, pp. 283-289. ISSN 1230-2821. DOI: https://doi.org/10.1515/ap-2015-0040 |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Acta Parasitologica | ||||||
Volumul 60 / 2015 / ISSN 1230-2821 /ISSNe 1896-1851 | ||||||
|
||||||
DOI:https://doi.org/10.1515/ap-2015-0040 | ||||||
Pag. 283-289 | ||||||
|
||||||
Descarcă PDF | ||||||
Rezumat | ||||||
The members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the Anopheles maculipennis complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of A. maculipennis among which: 58 A. atroparvus, 18 A. melanoon, 2 A. labranchiae, 52 A. maculipennis and 87 A. messeae. The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the A. maculipennis available in GenBank. The Species A. labranchiae is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the A. labranchiae sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country. |
||||||
Cuvinte-cheie animals, Anopheles, Base Sequence, cluster analysis, DNA, Ribosomal Spacer, insect vectors, Molecular Sequence Data, phylogeny, polymerase chain reaction, Romania, Sequence Analysis, DNA |
||||||
|