Introduction. Chronic musculoskeletal pain can have a significant impact on the quality of life and daily activities of post-stroke patients. Aim of the study. Estimation of musculoskeletal pain in post-stroke patients in the medical rehabilitation process. Material and Methods. Chronic musculoskeletal pain in 145 post-stroke patients was assessed. It was evaluated initially and after the rehabilitation interventions - pain according to the Visual Analogue Scale, the number of painful regions according to the Nordic Questionnaire and ADL according to the Barthel Index. Results. It was found that initially chronic musculoskeletal pain was presented by 145 patients (100%), of which 34 patients (23.4%) presented insignificant pain, 94 patients (64.8%) moderate pain and 17 patients (11 .7%) severe pain. After the rehabilitation interventions, the pain decreased in 74.9% cases, of which 57.2% cases presented moderate pain and 17.9% cases - insignificant pain. The number of painful regions being on average 4.782 regions, the standard deviation being 1.5010. After rehabilitation interventions, the number of painful regions decreased to 2,012 regions. Barthel index averaging 78.145. After rehabilitation interventions, it increased to 86,154. Conclusion. Medical rehabilitation can be an important component of the treatment of chronic musculoskeletal pain in post-stroke patients and can be useful in managing symptoms, improving function and quality of life.