Reinfection of tomato genotypes with tobacco mosaic virus or tomato aspermia virus results in a change of plant pollen productivity and male gametophyte activity. Under the influence of infection, the specificity of the genotype response for a number of functional traits of the male gametophyte was revealed. Under conditions of primary infection and reinfection, the genotype was a determining factor in the variability of pollen viability, while the change in the size of pollen tubes was mainly determined by the appearance of viruses. As a result of the experiments, two varieties Venets and Rufina were selected for further research, combining a high level of pollen viability and productivity. They testify to a high reproductive capacity of varieties; the combination of these indicators can be used as a tool for the selection of valuable genotypes.