Objective — to define a relationship of cyclic changes in the levels of sex hormones to autonomic disorders in patients with premenstrual syndrome. Subject and methods. A study group comprised 136 women with premenstrual syndrome and a control group consisted of 136 healthy women. The blood levels of follicle-stimulating hormone, luteinizing hormone, estradiol, progesterone, and testosterone were determined in phases I and II of the menstrual cycle. Autonomic nerve disorders were rated using an Autonomic Symptom Profile questionnaire on days 24—26 of the cycle. Results. In the study group, the level of sex hormones remained within the normal range although in that of estradiol and progesterone in the luteal phase was much higher in the patients with premenstrual syndrome than in the control women having no manifestations of the latter. The questionnaire scores were considerably higher in the study group than in the control one. The women with premenstrual syndrome during Phase II showed positive correlations with a direct strong relationship between estradiol levels and autonomic disorders and with a direct moderate relationship between progesterone levels and autonomic disorders. Conclusion. A direct correlation was found between blood estradiol and progesterone levels in phase II of the menstrual cycle and autonomic nerve disorders in patients with premenstrual syndrome.