This paper presents data on the current state of the collection of medicinal plants from Botanical Garden (Institut) of ASM, as well as the dynamics of taxa increase over the last decade, ways to raise and maintain the gene pool. The stages of biomorphological and phytochemical studies, the development and optimization of ecological cultivation technology, selection of forms valuable for breeding research were featured. Data on exsitu cultivation and research in various aspects of medicinal species with different rarity status, determining the optimal strategy for preservation perspectives are presented. The development of further goals including initiation of education programs in the collection is outlined.