Studies carried out in the last 25 years have shown that abiotic and biotic stresses have a negative impact on the male gametophyte quality and activity, including the ability to form pol-len and its germination, as well as the functionality of the pollen tubes [1; 3; 4]. The information about these processes is of particular interest not only through the influence on pollination and fertilization, but also for the application in the screening of valuable genotypes using of gametic methods. Considering the fact that, in many plant species, more than half of their genome is expressed at the pollen level, the male gametophyte can become a reliable system for evaluating the degree of interaction of the genotype with environmental factors [2]. The information regar-ding the associated action of viral phytopathogens and abiotic factors on the male gametophyte is quite limited in the specialized literature. The purpose of this research was to determine the reaction of the male gametophyte of tomato genotypes infected with viruses and their descen-dants to the action of high temperatures and hydric deficit.