Was studied the antimicrobial activity (antibacterial, antifungal) of the strain Streptomyces fradiae 19 isolated from chernozem soil of the Central Part of the Republic of Moldova. Study of the properties was carried out by using the test-cultures of opportunistic pathogens (E. coli, P. aeruginosa, E. faecalis, S. aureus, C. albicans) microorganisms and plant pathogenic bacteria (A. tumefaciens, C. michiganensis, X. campestris) and fungi (A. alternata, A. niger, B. cinerea, F. oxysporum, F. solani). Metabolites of studied strain showed the highest activity against the following test-microorganisms: S. aureus, C. albicans, growth inhibition zones ranged from 18.0 mm till complete inhibition in dependence of growing medium, but against phytopathogenic bacterium X. campestris, the diameter of the growth inhibition zones were from 24.5 till 32.0 mm.