Enoxil is a complex of biologically active substances obtained by
oxidation of grape seed tannins. It is a mixture of monomeric derivatives of
catechin, epicatechin in the free form and esterified with gallic acid as well as
with peroxidic compounds. Enoxil is characterized by high antioxidant activity
and amplified therapeutic properties. However, because of its high
hygroscopicity, there is a problem for its storage while saving the main
properties of the complex [1].
The aim of this work is to stabilize Enoxil in the matrix of polyacrylamide
(AQUALAN, Ukraine, synthesized for contact lenses). This hydrogel is
obtained by radical co-polymerization of monomers in the presence of
dispersion medium and represents the first type of modified hydrogels in which
the chemical bonds between the macromolecules are formed and the entire
volume of solvent between the cells is kept (up to 99 % of the total mass) so that
determines their thermal irreversibility, chemical inertness, resistance to changes
in pH over a wide range. Such hydrogels have high sorption properties [2].
To achieve the main goal, the polymer
was placed into the aqueous solution of Enoxil
(5 wt. %) for 10 h at 15 °C. The biomolecules
easily penetrated into polymer during adsorption
and were washed out during desorption. The
process of adsorption and the state of
biomolecules before and after adsorption have
been analyzed using spectrophotometric
measurements.
It has been shown that this method is suitable to integrate, stabilize and
provide a prolonged activity of biocomplex.
Acknowledgements
The authors are grateful to European Community, Seventh Framework Programme
(FP7/2007–2013), Marie Curie International Research Staff Exchange Scheme (IRSES
grant No 612484) for financial support of this project.
1. P. Kuzema, I. Laguta, O. Stavinskaya, O. Kazakova, M. Borysenko, T. Lupaşcu, Nanoscale Research
Letters. (2016) (DOI: 10.1186/s11671-016-1287-y)
2. Patent of Ukraine No 64849 “Biocompatible polyacrylamide hydrogel and method of its preparation”