Phospholipids as a component of the lipid spectrum represent the main part of structural integrity of sperm cell membranes. It is considered as natural fecundity factor which contribute to improving of spermatozoa cell membrane function by regenerative processes. Therefore, the purpose of the study was to examine the content of phospholipids, at various stages of a sperm cryopreservation. In results of the conducted researches is established that the content of phosphatidylcholine prevail above all fractions of phospholipids, which consistently decrease during cryopreservation. These changes are inherent to bull and boar spermatozoa. However, due to the fact that the content of phosphatidylcholine prevails over the other phospholipids, it is necessary to consider that this indicator can be the criterion of a fertility rate of spermatozoa which defines reproductive health of organisms relating to studied species.