Haploid selection of maize
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CRAVCENCO, Anatol, CLIMENCO (CRAVCENCO), Oxana. Haploid selection of maize. In: International congress on oil and protein crops, 20-24 mai 2018, Chişinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2018, p. 127. ISBN 978-9975-3178-5-6.
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International congress on oil and protein crops 2018
Congresul "International congress on oil and protein crops"
Chişinău, Moldova, 20-24 mai 2018

Haploid selection of maize


Pag. 127-127

Cravcenco Anatol, Climenco (Cravcenco) Oxana
 
Institute of Genetics, Physiology and Plant Protection
 
 
Disponibil în IBN: 17 septembrie 2019


Rezumat

Evaluation of pollen characters and elaboration of haploid selection methods is an important part of maize breeding. As the initial material 27 inbred lines (L1866, P101, P502, MK390, P523, B73, Mo17, W23, N6, L1362, P346, L459, P165, XL12, P346wx1wx1, Rf7, MK159, W47, 092, M11, A239, L276, 4nW23, A285, MK01, Co125, P343) and 19 F1 hybrids were used. The data were processed using the method of analysis of variance. Coefficients of correlation and regression were also determined. On the basis of the variability of such characters as “diameter of pollen grain”, “fertility”, “sterility”, “weight of pollen fractions” were elaborated the methods of adaptive genotypes selection, evaluation of attraction capacity, differentiation of genotypes in dependence of waterholding capacity. The highest values of pollen water-holding capacity were noted for Co125,MK01,P346,P523, M11, B73, 092 inbred lines and these genotypes can be used for the creation of drought resistant F1 hybrids. To accelerate selection for heterosis the method of genome compatibility was tested. It was found out that the nutrient medium supplied with the extract from pollen of A285 inbred line significantly stimulated pollen viability of MK390, N6, 4nW23. The extracts from pollen of N6 and 4nW23 genotypes significantly stimulated pollen viability of M11. This method can also help to evaluate combining ability of the genotypes. The heritability (h2) of pollen characters “fertility” and “diameter“ were studied and F1 hybrids with high values of these characters were found (P523xB73, L1866xA285, P343xRf7, L1866xF2, L1866xCo125 Co125xL1866). For inbred lines P165, Rf7, Mk01, P502, N6, P101, W47, Mo17, A285 (in combination with genotype P343) the heritability coefficient (for pollen fertility) was 0,987***. The high values of heritability of pollen fertility were noticed for genotypes F2, A285, P346, Co125 (in combination with genotype L1866) and for genotypes A239, B73, P502 (in combination with P523 genotype) - h2=0,75* and h2=0,981***, respectively. Inbred lines Mo17, L459, L276, P165, P502 had the highest values of pollen fertility. It was also shown that 11 inbred lines (P343, Co125, A285, 1362, 092, XL12, MK390, Mo17, P346, 4nW23, W47) have effective genetic systems of high adaptivity. The high values of pollen resistance to high and low temperatures were noted for L459, 092, P502 and these inbred lines can be used in cross combinations for obtaining adaptive F1 hybrids. For the determination of the genotypes resistant to low temperatures their pollen was stored in conditions of low (+1+80C) temperatures during 10 days. Analysis of variance showed that variability of pollen diameter (in normal and stress conditions) proved to be mostly genotype-depending (58,24%). Pollen diameter of inbred lines 4nW23, L459 differed insignificantly both in normal and stress conditions. These genotypes are likely to be stress tolerant. Thus, haploid selection for resistance to abiotic stresses is very efficient in maize breeding.