Optimizarea preparării probelor pentru analiza chimico-toxicologică a barbituricilor prin metoda HPLC. In lucrarea dată au fost studiate unele procedee simple de preparare a probelor de material biologic pentru dozarea ulterioară a barbituricilor prin metoda HPLC. Ca procedeu optim se propune deproteinizarea cu amestec metanol – 2 M H2SO4 (95:5). Acest procedeu in ansamblu cu analiza HPLC-UV asigură regăsirea barbituricilor cel puţin 97% din plasma sanguină şi o linearitate inaltă a dependenţei de calibrare in intregul diapazon studiat al concentraţiilor (pană la 160 mg/l) cu limita de cuantificare circa 1 mg/l

In the present work have been studied some simple procedures of biological material sample preparation for the following assay of barbiturates by the HPLC method. As an optimum way it is offered deproteinization by the mixture of methanol and 2 M H2SO4 (95:5). This procedure in combination with HPLC-UV analysis ensures recovery of barbiturates not less than 97% from blood plasma and high linearity of the calibration dependence in whole investigated range of concentrations (up to 160 mg/l) at limit of quantification about 1 mg/l..