Background: Defined daily doses remain as the most objective factor and can be considered as the cornerstone for making decision on determining the needs and organization of rational use of medicines, as well as for providing in practices suggestion of WHO Global Strategy that defines the use of antimicrobials in hospital treatment of patients. Material and methods: For this study we used data of 2009-2014 period, in the Emergency Medicine Institute of the Republic of Moldova which shows the dynamics of consumption of medicinal remedies of pharmaco-therapeutic group J – Antibacterials for systemic use of the Anatomical Therapeutical Chemical World Health Organization’s classification. Results: Total annual consumption of drugs group J – Antibacterials for systemic use in the evaluated period records a decrease from 662.2 DDD/1000 in 2009 to 464.1 in 2014 or by 29.9%, of which for parenteral use from 568.9 in 2009 to 346.9 or by 39% and for oral use from 93.28 to 117.20 or an increase by 25.6%. From total concumption the ratio between parenteral and oral use in the evaluated period varied from 85.9/14.1 to 74.8/25.2 percentage. Conclusions: The obtained data reveals that consumption of drugs group J – Antibacterials for systemic use in the evaluated period decreased significantly and in 2014 recorded a mean consumption of European hospitals..