In the Republic of Moldova during the last 40-50 years the quota of old autochthonous varieties in industrial vineyards permanently decreased and nowadays the majority of these varieties are present only in the Institute's Collection. During this time only an insignificant part of these resources were included in the research projects and, thus, their potential value for viticulture requires insights. The aim of the present work was the preliminary diversity evaluation of these varieties. Thirty-six cultivars were described according to the primary and secondary ampelographic descriptors, and to the phenotyping protocols for eno-carpological characterization. Based on the obtained data and on literature, some characteristics were established such as classification into Negrul's eco-geographical proles, berry colour, agro-biological properties and resistance to abiotic factors and pathogens.