In the last years a special attention is given to the researches of the ergosterins yeasts synthesis. The yeasts of the Saccharomyces genus represent the main producer of ergosterin (pro-vitamin D2). Its concentration can achieve till 10% from the dry weight of biomass. The present work is dedicated to the study of the regularities of the lipids and sterins biosynthesis of Saccharomyces carlsbergensis CNMN-Y-15 yeasts (the stem is patented as an active producer of sterins), cultivated in nourishing environments with different sources of azoth, carbon, predecessors and inductors and with the aim of their optimization for the directed synthesis of ergosterins. In conditions of yeasts periodical cultivation it was determined that from 4 researched sources of azoth and carbon, the culture is metabolizing more effectively the melass and glucose. The melass promotes the active growth of the biomass (till 7,06 g/l), but with a loss of the lipids and sterins biosynthesis. The maximum content of lipids (till 14,4 … 16,8% in dry weight) and sterins (till 9,5 … 10,1% in dry weight) was discovered at the yeasts cultivation in the presence of glucose in concentration of 20-40 g/l. An important influence on the yeasts biosynthetical activity is played by predecessors. It was determined that zinc acetate in concentration of 30 mg/l promotes the active accumulation of the biomass. The stimulating effect of the biosynthesis of oleogenic biologically active substances is revealed at the yeasts cultivation in the presence of manganese acetate, which in concentration of 10 mg/l is stimulating both the biosynthesis of lipids (to 38%) and sterins (to 21% in comparison with the control data). The positive effect can be explained by the fact that the acetates are proven to be activators of the Ko-A coenzyme, which is necessary in intermediate reactions of the biosynthesis of isoprene junctions. For the 1st time it was determined the efficiency of the zinc, manganese and chrome coordination junctions’ application as inductors of sterins biosynthesis at yeasts. In the process of evaluation of coordination junctions’ influence it was determined that the junctions of zinc and manganese represent the most effective inductors. The junction [Zn(L-ala)2] in concentration of 15 mg/l is stimulating the yeasts productivity with 55% in comparison with control data. In the case of application of [Mn(Gly)2Cl2] in concentrations of 5-10 mg/l the sterins content in the yeasts biomass is rising to 16,8 ... 18,6 in comparison with control data. As the influence of the sources of azoth, carbon, predecessors and inductors on biosynthetical processes of yeasts had been studied separately, it is interesting to determine the effect of the influence of the totality of these factors both on productivity and sterins biosynthesis and in this regard to optimize the nourishing environments. There were elaborated more than 20 kinds of nourishing environments. According to mathematical planning of the experiment it was optimized the composition of two nourishing environments for the cultivation of Saccharomyces carlsbergensis CNMN-Y-15, which allows the increasing of the productivity and the content of sterins in the yeasts biomass. The nourishing environment in the composition of which additionally are included melass and zinc coordination junction allows us to obtain till 8,36 g/l of dry biomass, that represent with 89% more in comparison with the control nourishing environment. The other optimal nourishing environment in the composition of which are added glucose and manganese acetate give us an increase in sterins (with 80,8% in comparison with control data). The optimal nourishing environments that were elaborated are recommended to be used in the biotechnology of Saccharomyces carlsbergensis CNMN-Y-15 yeasts stem cultivation as sterins producer