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SM ISO690:2012 COJOCARU-TOMA, Maria, COTELEA, Tamara, KULCIŢKI, Veaceslav, UNGUR, Nikon, CIRIMPEI, Octavian, NACU, Viorel, JIAN, Mariana, COBZAC, Vitalie, ORGAN, Adina. Phytochemical study and antioxidant action of Lavandulae angustifoliae residues. In: New frontiers in natural product chemistry.: A destiny on the altar of research. Dedicated to academician Pavel Vlad, Ed. 6, 21 mai 2021, Chișinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2021, Ediția 6, p. 17. ISBN 978-9975-3336-7-2. DOI: https://doi.org/10.19261/nfnpc.2021.ab10 |
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New frontiers in natural product chemistry. Ediția 6, 2021 |
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Conferința " New frontiers in natural product chemistry." 6, Chișinău, Moldova, 21 mai 2021 | |||||||
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DOI:https://doi.org/10.19261/nfnpc.2021.ab10 | |||||||
CZU: 582.949.26+543.544+615.01 | |||||||
Pag. 17-17 | |||||||
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Lavender industrial processing involves steam distillation of oil and vegetal residues are normally discarded unvalorized. Lavender wastes have been paid little attention from the point of view of secondary metabolites recovery. The available literature date report mostly on the quantification of phenolics and evaluation of antioxidant potential in crude or fractionated extracts [1]. Few data are available on the influence of pretreatment procedures of vegetal raw material on extraction yield, while triterpenic acids are totally overlooked in the characterization and application schemes [2]. The current work presents the chemical analyses of 2 lavender residues with different extraction methods and pretreatment procedures. The extraction yield and content of the secondary metabolites was evaluated. The content of ursolic and oleanolic acids has been demonstrated both chromatographically (TLC, GC-MS) and spectroscopically (NMR). The total phenolic content was determined by the Folin-Ciocalteu method and flavonoids (based on aluminum chloride complex) and measured by UV-visible spectrophotometer. Antioxidant activity was determined by three methods: DPPH, ABTS and metal chelating activity [3]. The results confirms that the residues can be used as a source of polyphenols and the Lavandulae R-1 residue has a higher content of polyphenols expressed in gallic acid (2.19%) and flavonoids expressed in rutin (2.03%), followed by Lavandulae R-2 residue, with a polyphenol content (1.12%) and flavonoids (1.43%) in correlation with the antioxidant action (Table 1). Lower absorbance of the reaction mixture indicated higher free radical activity for Lavandulae R-1 residue. Table 1. Antioxidant activity of Lavandulae residues. Samples DPPH, IC50 μg/ml ABTS, μM TEAC/g Metal chelating activity, μM/g Lavandilae R1 96.651±0.121 27.556 ±0.012 75.145±0.016 Lavandulae R1 103.241 ±0.132 32.267±0.023 71.062±0.021 Trolox 5.032 ±0,003 EDTA 98.66 |
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