The scientific paper presents the results of research of productive and reproductive parameters of cows of Holstein breed of Dutch and German origin in the herd J-S.C. „Aydyn”, Comrat Administrative and Territorial Unit Gagauzia. For the analysis was used performance control for the year 2015: cows on the first lactation (n=122) and the second lactation (n=43) of Dutch origin and cows on the first (n =129) lactation – of German origin. It was established that from cows of the Dutch origin for the first lactation on average was received 7803 kg of milk, which is with 589 kg more than from the cows of German origin, a highly significant difference (P <0.001). It is established by the analysis that 50,8% of the animals of Dutch origin had the productivity from 7501-8000 kg to 9001 kg, whereas 70,5% of the animals of German origin had the productivity from 6000-6500 to 7001-7500 kg and only 29.5% from 7501-8000 to 9001 kg of milk per lactation. The average length of the dry period at cows of Dutch origin was by 6.5 days less than at the cows of German origin, the difference is authentic at P <0.05. Service period at cows of Dutch origin averaged 219.9 ± 12 days, the German origin – 168.2 ± 15.4 days. This affected the increase of the between calving interval at the animals of Dutch origin (483 days), the German origin (446 days), which exceeds the desired duration by 118 and 81 days, respectively.