Phenolic compounds have been the subject of intense research in recent years due to their possible beneficial effect on human health. The phenolic compounds are secondary plant metabolites contained within the skin, seed, and flesh of grapes and are extracted into wines during the process of vinification. One of the major groups belonging to phenols is represented by anthocyanins. The best properties known of these secondary metabolites is their strong antioxidant activity in metabolic reactions due to their ability to scavenge oxygen radicals and other reactive species. The future makes phenols and especially anthocyanins a potential tool for use in studies on oxidative stress, the aging process and cancer, especially since it has been reported that anthocyanins inhibit the growth of cancer cells and act as chemotherapeutics for numerous diseases. Plant cells/tissue cultures offer an opportunity for continuous production of these plant secondary metabolites and especially that obtained from the major known source of anthocyanins, the familiesVitaceae. The aim of this experiment consists in emphasizing the correlation between biomass accumulation and biosynthesis ofthese important secondary metabolites necessary to improve the capacity of Vitis vinifera L. cvs. Negru Vartos callus culture to accumulate phenols, respectively anthocyanins and to become a valuable source for these secondary metabolites with a broad spectrum of pharmacological and therapeutic effects. The study was conducted using a primary callus culture of Vitis vinifera L. cvs Negru- Vartos initiated from leaf fragments of in vitro plantlets and subcultivated on a basal Gamborg- B5 (1968) medium supplemented with 0.1 mg/l NAA (α- naphtalene acetic acid), 0.2 mg/l kinetine, 2 g/l casein hydrolysate, 30 g/l sucrose, 8 g/l agar (Difco). Within a period of 31 days was monitored the growth response of Vitisvinifera L. cvs. NegruVartos callus, subcultured on fresh medium each week and colorimetric determination of total phenolics as simple, rapid and reproducible assay of the whole complex of substances possessing antioxidant activity in order to accesses the potential of thiscallus as in vitro producer of bioactive substances. The values of total monomeric anthocyanins confirm that the high accumulation in the fourth week of the phenolic compounds is mainly represented by the anthocyanins. But in the same time the values recorded in the last week of cultivation reveal a significant accumulation of polymerized/browning anthocyanins which can suggest that after these four weeks of callus cultivation could start a phase of development represented by a decrease in anthocyanin accumulation. Our study revealed that high rates of biomass production don’t have negative effects on the production of this secondary metabolites, the results obtained being an exception for the published literature reports as far as secondary metabolism in vitro concerned. This experiment represents the basis of future researches aiming the optimum developmental stage of Vitis vinifera L. cvs. Negru Vartos callus for the improvement of phenols, respectively anthocyanins accumulation and for demonstrating the potential of this experimental system for food, pharmaceutical and cosmetic industries.