Kiwifruit (Actinidia chinensis Planch) is a dioecious woody species originated from China and introduced in New Zeeland in the beginning of the century. Nowadays kiwifruit is an important crop plant around the world. The increasing interest in kiwifruit has been followed by increased research on this plant. For commercialization of this crop throughout the year and to meet the growing demand for fruit in vitro culture techniques are being used as alternative method for propagation in many countries. In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit, using different organs as well as leaf blades, petiolesand stem under in vitro conditions. For this purpose the different medium (MS-Murashige-Skoog, 1962; S 2,5 - Standardi A. 1983; B5 - Gamborg, 1968) were tested. Effects over the morphogenic capacity of the explants were tested using different auxin (IBA, ANA, 2,4-D) cytokinin (BAP and zeatine) and gibberellins (GA3) concentrations. The leaf explants, petiol and stem were used to initiate callus formation. The first seven days explants were kept in the dark and then transferred in the culture room. The cultures were incubated at a temperature of 24±1°C under 16-h photoperiod with illumination by white fluorescent light with an intensity of 2000. The callus formation started about two weeks after inoculation. Callus induction was optimum on the S 2,5 medium in the presence of the 0,2mg/l ANA and 1,0 mg/l zeatine for all types of explants. The frequency of callus derived from petiole (81,48%) was higher than those from leaf blades (69,46%). Two types of callus were distinguished: morphogenetic and nonmorphogenic. They differed not only in their capacity for organ formation, but also in their structure, appearance and morphology. Histological studies of calli segments confirmed this fact. The use of zeatin+auxin combination seems to be very effective in the culture medium for callus induction and followed by shoots regeneration. Organogenesis in vitro depends on the application of phytohormones and also on the ability of the tissues to respond to these hormonal changes during culture. Specifically, the presence of auxins and cytokinins is necessary for indirect organogenesis. After 3-4 month of cultivation callus were subcultured into regeneration medium. The first shoot formation was appeared in S 2,5medium containing 1,0 mg/l BAP and 1,0 mg/l GA3. This medium is considered to be more effective for shoot formation and plant regeneration. Organogenetic capacity was varied in very large limits depending especially on type of explants, culture media and hormonal composition. Histological examination revealed the presence of meristematic centers in morphologic callus after one month of culture on regeneration medium. These centers consisted of meristematic cells with regeneration ability. Cells with numerous starch grains were presented adjacent to these centers. Transferring regenerated shoots without roots to new rooting medium produced whole fertile plants. To induce rooting, have been used more possibilities. Firstly, shoots 4-5 cm long were excised from the callus and the cut end was placed in MS rooting media in the presence of BAP and IBA. In other case for root induction was used ¼ MS liquid medium added with only IBA.Finally, excised shoots were soaked in a solution of IBA (750 mg/l) during the 10 şi 15 seconds.The best root formation was atested on the MS medium withBAP and IBA. In conclusion is important to note that leaf and petioles explants offer the possibility to produce plants through indirect organogenesis of kiwifruit, after intensive proliferation of callus. The obtained results reaffirm the fact that the Actinidia chinensis show a good organogenetic capacity that varies depending on the type of explant, hormonal balance and the culture medium.