One of the key challenges of the modern economic development is elaboration of the highly efficient innovational technologies and equipment for energy production. A certain contribution with this regard is made by bio-power engineering based on the use of agro-industrial wastes and linked with the production of biomethane, biohydrogen and other energy bearers. Thus, anaerobic digestion of biomass with biogas production is broadly applied throughout the world, however, as a rule, methane contents in biogas usually makes 60-70%, and that of biohydrogen – no more than 20%. In addition, conventional processes have low productivity and fermentation cycle makes 3 to 10 days and more. At the same time, enzymatic catalysts available, applied to accelerate these processes, are costly and insufficiently efficient.     The search of principally new biostimulators have revealed the new direction – using of biologically active substances (BAS) as phyto-catalysts. The efficiency of digestion processes was found to be increased essentially, once these substances have been introduced in micro-amounts into the fermented biomass. Some BAS are in the fine nano-structured state, affecting the microorganisms development and promoting their accelerated growth.     The studies of the biomass (alcohol vinasse as a waste) anaerobic fermentation, using some substances of phenol nature as phyto-catalysts in concentrations of  10-5 ÷ 10-3 % with regard to the biomass contents, made it possible to estimate the dehydrogenase activity, to reduce the lag-phase and activate the exponent growth of microbial community, essentially increasing the stationery phase of its development. It was shown that using of the proposed BAS increases the biogas process rate by 2,5-3 times and enhances the coefficient of biomass anaerobic fermentation by 15-20 %. At the same time, methane contents in biogas composition can reach up to 85÷92%. Accordingly, the biogas caloric value increases by 31-36 %. The biochemical process of anaerobic digestion was carried out under the mesophyl conditions at the temperature of  33 ± 2оС. The summary contents in biogas emitted was determined with regard to the removed COD during the 4 and 7 digestion days.      It is supposed that BAS promote the intensification of anaerobic digestion of organic substrate due to their anti-oxidant, anti-hipoxanth and anti-mutagen activity. BAS stabilize the cell membranes of microorganisms, reduce the peroxide oxidation of lipids and prevent the deterioration of cell membranes.   The studies of the isoprenoid BAS effects on the stimulation of biochemical digestion processes appeared to be interesting and important. It was shown that these substances inhibited the methanogenic process, while stimulating the emission of biohydrogen. The microorganisms’ consortium was found to be capable to reduce protons, getting rid of the reducer excess. As a result, predominant emission of Н2, СО  and СО2 occurred. These processes, taking place under anaerobic conditions at the initial acetogenic stage, are accompanied with the formation of a series of intermediate fermentation products. The contents in the molecular hydrogen in the componence of biogas, in dependence on the functional groups of BAS tested, reached  67,8 and 40,2%. The mechanism of appropriate biochemical processes is considered.