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SM ISO690:2012 REVENCU, Tatiana, TRIFAN, Valentina, NACU, Ludmila, TAGADIUC, Olga, SMESHNOI, Valentina, NACU, Viorel. The influence of certain obstetric factors on the collection, isolation and quality of cord blood units. In: Nanotechnologies and Biomedical Engineering, Ed. 2, 18-20 aprilie 2013, Chișinău. Technical University of Moldova, 2013, Editia 2, pp. 539-544. ISBN 978-9975-62-343-8.. |
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Nanotechnologies and Biomedical Engineering Editia 2, 2013 |
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Conferința "International Conference on Nanotechnologies and Biomedical Engineering" 2, Chișinău, Moldova, 18-20 aprilie 2013 | |||||||
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Pag. 539-544 | |||||||
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The umbilical cord blood is a rich source of hematopoietic and mesenchymal stem cells and it can be used for cellular therapy. This article presents data taken from 50 cord blood samples (46 in utero, 4 ex utero). The nucleated cells were isolated from the cord blood using a dextran solution. The quantity of stem cells having the immunophenotype CD34+, CD45+ and CD90+ was determined, using flow cytometry. The nucleated cells were cultivated in a DMEM (DulbeccoModified Eagle Medium) and biochemical and cytogenetic research was later performed. |
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Cuvinte-cheie stem cells, cellular therapy, mesenchymal and hematopoietic stem cells, (umbilical) cord blood, flow cytometry. |
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