The influence of certain obstetric factors on the collection, isolation and quality of cord blood units
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REVENCU, Tatiana, TRIFAN, Valentina, NACU, Ludmila, TAGADIUC, Olga, SMESHNOI, Valentina, NACU, Viorel. The influence of certain obstetric factors on the collection, isolation and quality of cord blood units. In: Nanotechnologies and Biomedical Engineering, Ed. 2, 18-20 aprilie 2013, Chișinău. Technical University of Moldova, 2013, Editia 2, pp. 539-544. ISBN 978-9975-62-343-8..
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Nanotechnologies and Biomedical Engineering
Editia 2, 2013
Conferința "International Conference on Nanotechnologies and Biomedical Engineering"
2, Chișinău, Moldova, 18-20 aprilie 2013

The influence of certain obstetric factors on the collection, isolation and quality of cord blood units


Pag. 539-544

Revencu Tatiana, Trifan Valentina, Nacu Ludmila, Tagadiuc Olga, Smeshnoi Valentina, Nacu Viorel
 
”Nicolae Testemițanu” State University of Medicine and Pharmacy
 
Proiecte:
 
Disponibil în IBN: 21 iunie 2019


Rezumat

The umbilical cord blood is a rich source of hematopoietic and mesenchymal stem cells and it can be used for cellular therapy. This article presents data taken from 50 cord blood samples (46 in utero, 4 ex utero). The nucleated cells were isolated from the cord blood using a dextran solution. The quantity of stem cells having the immunophenotype CD34+, CD45+ and CD90+ was determined, using flow cytometry. The nucleated cells were cultivated in a DMEM (DulbeccoModified Eagle Medium) and biochemical and cytogenetic research was later performed.

Cuvinte-cheie
stem cells, cellular therapy, mesenchymal and hematopoietic stem cells, (umbilical) cord blood, flow cytometry.