Seed formation in Torreya grandis fortune ex lindl. Introducted to the southern coast of Crimea
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RUGUZOVA, Anna. Seed formation in Torreya grandis fortune ex lindl. Introducted to the southern coast of Crimea. In: Conservation of plant diversity, Ed. 3, 22-24 mai 2014, Chișinău. Chișinău: Gradina Botanica (Institut), 2014, Ediția 3, pp. 110-111. ISBN 978-9975-62-370-4.
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Conservation of plant diversity
Ediția 3, 2014
Simpozionul "Conservation of plant diversity"
3, Chișinău, Moldova, 22-24 mai 2014

Seed formation in Torreya grandis fortune ex lindl. Introducted to the southern coast of Crimea


Pag. 110-111

Ruguzova Anna
 
Никитский ботанический сад
 
 
Disponibil în IBN: 20 mai 2019



Teza

Introduction Plants` introduction is a very important part of botanical investigations. But if fi rstly the main task was simply to grow plants in the new conditions, now more attention is paid to the creation of the so called “introductive populations”. For creation of viable populations we need plantings from seeds have been formed in the new growing conditions. At the same time seed formation is a very complex process that includes number of stages and for many plants especially gymnosperms it is long in time. Investigations of many species have shown that embryological processes controlled not only with the organism`s features but they are also under control of the environmental factors. Thus the studies of plants sexual reproduction in the new growth conditions from one hand let us to decide a practical problem of vital seeds obtaining and from the other hand give us more information about adaptive potential of plant species. Material and methods Torreya grandis Fortune ex Lindl. is a dioecious tree native for some regions of China (S Anhui, N Fujian, NE Guizhou, W Hunan, S Jiangsu, N Jiangxi, Zhejiang). Usually grows in mountains, open valleys, often by streams, on yellow, red, and dark soils; 200-1400 m. In Arboretum of Nikitsky Botanical Gardens (NBG-NSC) there are two trees of T. grandis – male and female. Both form the reproductive structures. We made phenological observation during the two years. Material for embryological studies was fi xed in ethanol: hlorophorm: acetic acid (6:3:1) and kept in 70% ethanol. The material was dehydrated through a graded ethanol series and fi naly imbedded in paraffi n. Sections were cut at a thickness 10-12 microns. Series were stained with methyl green-pyronin combined with alcian blue. Analyses were made under light microscopy “Jenaval“. Results and discussions It has been determined that on the Southern coast of Crimea in T. grandis the great number of male and female cones are formed every year. Microstrobili are single on the top of the short shoots, bearing 25-30 mycrosporophylls with 2-4 microsporangia each. At the time of pollination mature pollen grains are twocelled. Pollen viability is 70-82%. Female reproductive structures (megasporangia) are also single but mostly are in pairs on the common postament in the axil of reduced leaf. Pollination may takes place in I – III decades of May, depends from weather conditions. To the moment of pollination development of female structures is successful and mature ovule has nucellus and integument which forms short micropilar channel on the top of which pollination drop is. At the time of pollination female gametophyte is on the stage of microspores mother cell. There are some evidence that in the ovule of T. grandis more than one microspore mother cell are formed but we haven`t observe this. Only successfully pollinated ovules continue their development, other degenerate. Further development takes about 15 months and seeds are ripening in November the next year after pollination. In our Arboretum we have a lot of seeds but the problem is that in mature seeds embryo is still immature and it needs stratifi cation which is impossible under the weather conditions of the Southern coast of Crimea. Conclusions According to the results of our investigations we consider that T. grandis has strong potential for viable seeds formation in the conditions of the Southern coast of Crimea. But investigations for stratifi cation condition for seed are needed for getting plantlets.