Introduction Open-air village museums represent objectives with a special status because they bring together monuments represented by buildings that are no longer functional, having just the role of museum exhibits into the direct incidence of the environmental factors. The biotic factors can be considered essential for the process of wood biodeterioration due to the fact that the wood is an important source of organic matter and mineral elements for different organisms that alternate according to the deterioration of wood matter in time. Material and methods The collection of sporiferous bodies was made following periodical investigations of wood to all 16 exhibits from Bucovina Village Museum and 21 exhibits from Village Museum - Sighetul Marmatiei. For each sample collected from the construction wood have been made observations recorded into worksheets: the affected building element, position of sporiferous bodies above the ground, wood type, degradation degree, moisture of wood. The identifi cation of the species was made based on the analysis of the morphological macroscopic and microscopic characteristics (1, 2). Results and discussions Our investigations highlighted the diversity of macromycetes involved in biodegradation of the monuments from the Bucovina Village Museum - Suceava and Village Museum - Sighetul Marmatiei, and allowed the identifi cation of the causes that increase the installation and the development of fungi. We proceed to an estimation of extension and degree of the attack on the main elements of the constructions. A comparative study was made regarding the diversity of fungi into the studied open air museums, and was highlighted the frequency of some species of macromycetes as important agents for wood biodeterioration: Schizophyllum commune, Gloeophyllum sepiarium, G. abietinum, Fibroporia vaillantii, Phellinus ferruginosus, P. contiguus, Hyphodontia arguta, Dacrymyces stillatus. The most damaged areas are those from the upper side of the constructions, caused by malfunctions at the general water drainage system at the level of the roof, itself strongly affected. In the lower side of the constructions have been identifi ed macromycetes as well as degradable elements, both on the inside and the outside (3). Conclusions On the construction wood in the both open air museums were identifi ed 28 species of macromycetes. Species as Gloeophyllum sepiarium, Stereum hirsutum, Schizophyllum commune were reported very commonly, a situation explained by incidental environmental factors and growth conditions similar to those from the natural forest ecosystems.