Tayberry is a hybrid obtained by the crossing of the blackberry - Rubus fruticosus and the raspberry - Rubus idaeus. The original plant was selected from a family of seedlings resulting from a cross made in 1969 at the Scottish Horticultural Research Institute, Dundee, UK, between the octoploid blackberry Aurora and a tetraploid raspberry 626/67. This variety resembles the Loganberry in some respects, but is superior to it with respect to fruit size, yield, fruit color, mode of presentation of fruit. In vitro culture of Tayberry is cost effective for obtaining vitroplantlets free of viruses in a short period of time. In this paper we have presented some aspects concerning the in vitro propagation and the evaluation of different concentrations of growth regulators BAP and NAA. The culture media were prepared using the stock solution of macro-, microelements and vitamins, subsequently being autoclaved at 100 ° C for 5 min. For the in vitro micropropagation, it was used Murashige Skoog  medium [3] supplemented with growth regulator BAP. The sucrose in the culture medium may be replaced with sugar 30 g/l. As study material of the cultivar Tayberry in  in vitro culture served cauline meristem (apical meristem). The explants were inoculated immediately after shaping and disinfection, as it degrades rapidly oxidizing. First, it is prepared a small number of explants. The speed of degradation and loss of viability depend on the nature of explants, on their size and the conditions in which they are kept (on the glass, filter paper moistened with distilled water or are submerged in water). The inoculums came from open ground after being sterilized according to the  methods described by Cachiţa-Cosma [1] and modified in the laboratory. After the investigation on the multiplication of variety Tayberry on these mediums, it was observed that efficient result was obtained on MS medium with the addition of BAP for a period of 55-60 days. It was found that on the medium supplemented with BAP (0.3 mg / l) the number of adventitious shoots is about 14, but with the increase of amount of BAP in the culture medium increases the number of adventitious shoots. In the culture medium supplemented with BAP at a concentration of 0.7 mg / l their number reaches 26 shoots. With the increasing of number of adventitious shoots decreases their length. For Tayberry, it was established an optimal culture medium for rootedness, with the composition – MS 50% supplemented with 0.1mg / l NAA. On this substrate it has been observed a growth of the plantlet axis of about 8.0-10.0 cm, over a period of 30 days, and the basal part of the plant having a branched root system, composed of 5-6 white, branched rootlets. The plant being of an increased vigor, having a high potential of growth, afterwards being micropropagated on the culture media, and the basal part of the shoot is passed to ex vitro. Simultaneously was tested  MS 50% medium without growth regulators. It was established that on this culture media rootedness process is slower (60-65 days), and the development of the aerial part is harder. The plant does not grow more than 2-3 cm in length. So we conclude that:  Tayberry cultivar showed a positive assertion in multiplying by vitro cultures. Optimal culture medium for the development of plantlets for microcloning (larger number of shoots, more vigorous, that are long enough) is MS supplemented with BAP (0.5 mg / l). Basic culture medium for the rootedness process is MS 50% supplemented with NAA (0.1 mg / l).