Introduction P53 tumor suppressor gene is considered one of the most important genetic biomarkers for colon cancer. Detection of P53 at early stages of this disease can help for improving the outcomes for patients. Consequently, it is very significant to create a sensitive sensor for assessment of P53. Experimental Proposed amperometric sensors were design in the laboratory for the determination of P53. The sensors were based on graphite and graphene powder with electro active modifiers such as porphyrins and oleamides. Results and discussion Very low concentrations of tumor suppressor gene were made by utilizing the proposed amperometric sensors. These limits are in the range of early detection, but can also allow the assay of P53 in stages 3 or 4 of the colon cancer. The sensors showed excellent sensitivity when compared with others colon cancer biomarkers. But also over other components from whole blood such as neurotransmitters. The recovery tests performed proved the convenient of these sensors for the early detection of P53 in whole blood samples. Conclusions The determination of P53 by using the proposed amperometric sensors based on physical immobilization of porphyrins and oleamides in graphite and graphene based pastes, revealed excellent results that can be used as a reliable method for the assessment of P53 in whole blood samples.