The aim of this study is to analyze the biosynthesis conditions, structure and the biological applications of an exopolysaccharides (EPS) producing strain, isolated from Romanian wheat flour. The strain was shown to produce a high amount of EPS (7.4 g/L of freeze-dried EPS/L culture medium) in MRS culture medium improved with sucrose and dissolved in UHT milk. The extracted and purified EPS were characterized by FTIR, NMR, GPC, DSC and TGA analysis. To assess the cytotoxicity, the CellTiter 96sAQueous One Solution Cell Proliferation Assay (Promega) was performed on normal human dermal fibroblasts, NHDF (PromoCell)1. The antifungal activity of the EPS was assessed on Candida albicans growing as planktonic and biofilm phase. The in vitro susceptibility testing was performed following the EUCAST EDef 7.2 guidelines2. The antimicrobial activity was measured by the agar disk diffusion method using three different reference strains, which are common and virulent pathogen agents met in care-associated diseases. Due to their characteristics, these exopolysaccharides are suitable as ecological hydrophilic matrix for controlled release of drugs in pharmaceutical industry.