Phytoplasmas are a wall-less, non-cultivated in vitro, phloem colonizing bacteria which infect more than 700 plant species. Many of these species are economically important: tomato, potato, maize, grape and others. Phytoplasma infection causes a huge disturbance in growth like phylody, sterility of flowers, proliferation of lateral buds giving a bushy aspects, dwarf. Phytoplasma is mainly transmitted by insect vectors as planthoppers, leafhoppers and psyllids. Also phytoplasma may be transmitted through weeds like bindweed (Convolvulus arvensis and Calystegia sepium) and stinging nettles (Urtica dioica) which are natural reservoirs for insect vectors. Although this infection can be spread by vegetative propagation such as grafting, rhizomes, or bulbs but it remains unclear the possibility of phytoplasma transmission thought seeds.  Our goal was to check phytoplasma infection in tomato seeds and seedlings.  In our research seeds of three tomato genotypes, Elvira, Cerasus and Deşteptarea, were analyzed. DNA isolation was performed by alkaline expess method. 2/3 part of a seed was taken for one sample. To identify the infection of tomato seeds we used two primer pairs specific for Ca. P. solani, which allow nested-PCR analysis. Electophoresis was carried out in 2% agarose gel, the buffer TBE × 1, bands visualization was performed using EtBr. Results of the molecular diagnostic of untreated seeds revealed the presence of Ca. P. solani. The infection has been determined in 42, 9 % of all analyzed samples. However, nested-PCR analysis showed the lowest number of infected with phytoplasma seeds in variety Cerasus. This may indirectly indicate that the variety Cerasus is more resistant to Ca. P. solani compared with Elvira and Deşteptarea, taking into account a lower part of the infected seeds. Then we identified the presence / absence of phytoplasma infection in tomato seedlings of three tomato genotypes: Elvira, Enigma and Uspeh x L325, which were grown in thermostat. The infection has not been determined. The quality of DNA was tested using UMD 2629 primers specific for tomato pathogen related gene. Amplified fragments UMD 2629 have been detected in all analyzed samples that demonstrate the high quality of isolated DNA.  Thus, the results of these experiments confirm the assumption that phytoplasma is not transmitted by tomato seeds.