This article describes the influence of urbanization process on forming of river water resources in the Republic of Moldova, being based on application of methodological recommendations presented in the normative document D.01.06- 2012, that regulates design and construction of water supplies system in the rural and urban areas. Anthropological influence in the annual flow index in urban areas is set as a function of the specific area of urbanized land. The significant increase in anthropological influence index (kUr> 1.20) is linked with the landscape regions, within which large towns (Chisinau, Balti, Straseni, Calarasi, etc.) The minimum value of 1.08 is observed within the boundaries of Cahul Plains. The growth of annual runoff due to urbanization of watersheds is considered permissible in case when kUr does not exceed1.10. Parts of urban areas with high level of storm waters causes high slope runoff, leading to flooding of settlements that cause considerable damage.