Heavy metals (e.g., Hg, As, Pb, Cd, and Cr) can accumulate and migrate in soil environments and due to their cumulative effects or long-term interactions can effects in the negatively way the crop yield and its consumers by entering in human bodies by the food contamination. The results can be observed in an increased incidence of chronic diseases (deformity of body or various types of cancer) and can disturb human metabolomics, contributing to morbidity and even mortality. Food security became thus a high-priority concern for sustainable global development. [1] Scientific papers revealed that fruits and vegetables consumption is the primary pathway of human exposure to heavy metals and recommended to never using sewage wastewater to irrigate vegetables unless it is obligated. [2] They reveals that the untreated wastewater is the primary source of soil pollution, due to contaminated water containing high amounts of heavy metals and microorganisms and lead to increase the concentration of metals and pathogens on the lettuce. Using wastewater for a long time, this may lead to severe risk to consumer‘s health. The aim of the present work consists of the evaluation of the heavy metals impact on bioactive compounds (e.g. assimilating pigments, polyphenols) from lettuce. The heavy metals taken into account were: Pb, Mn, Cu, Zn, Ni, Cd, having 3 different concentrations: one under accepted limit, one maximum accepted limit and one above maximum accepted limit. The growth of plants in the presence of different concentrations of the heavy metals salts was compared with those grown in the absence of salts. The influence of heavy metals salts on bioactive compounds and elemental content of lettuce were investigated by UV-Vis spectrophotometry. The correlation between the content of metals and biologically active compounds has also been traced.