Throughout the world, municipal, agricultural and industrial waste discharges into the environment are ubiquitous, which means that antibiotics can be found in significant concentrations in many rivers, sediments and soils. Because antibiotics and antibiotic-resistant bacteria come from the same source, they are often found together. Due to the incomplete removal of drugs at treatment plants, as a result of their discharge into wastewater, they are released into surface water. Purification of these waters is carried out by different methods, usually in a complex manner, which significantly increases costs. The high price of purified natural water leads to the need to find cheap and effective materials for water purification. Recently natural zeolites have been used for solving practical water purification problems. The adsorption method using natural zeolites is characterized by the absence of secondary contamination, cost-effectiveness associated with the possibility of multiple use of the sorbent. This study contains data on the adsorption of β-lactam antibiotic ceftriaxone (CEF – C18H18N8O7S3) from the group of cephalosporins from aqueous solutions on natural zeolites clinoptilolite and mordenite and their acid-modified forms. Acid modification of natural zeolites leads to an increase in the sorption activity of zeolites, which is associated with an increase in the pore size as a result of unblocking the channels of the aluminosilicate framework of the sorbent, which contributes to a greater accessibility of pollutants to active sites. The study of the adsorption capacity of the studied samples to ceftriaxone was performed under dynamic conditions on a flow-through installations. The change in the concentration of the antibiotic at the outlet of the adsorption column was determined by HPLC. The main information about the work of the sorbent layer at different parameters was obtained by plotting the output curves (isoplan) in the С C0- t coordinates. The experiments were performed until the zeolite layer was completely worked out (until the concentration of water behind the sorbent layer appeared to be equal to the concentration in the initial mixture C0). Analysis of the experimental data obtained showed that the best characteristic adsorption parameters were possessed by samples of mordenite and clinoptilolite modified with 5N HCl solution. It was established that acid-modified forms of zeolites are effective, competitive adsorbents for removing frequently used antibiotics from wastewater. It is very important from the economic point of view that it is possible to reuse worked out sorbents after their regeneration.