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SM ISO690:2012 TARNA, Carmelia, CALALB, Tatiana. Comparative study of alkaloids in species of genus Galanthus from the Alexandru Ciubotaru National Botany Garden. In: Cercetarea în biomedicină și sănătate: calitate, excelență și performanță, Ed. 1, 20-22 octombrie 2021, Chişinău. Chișinău, Republica Moldova: 2021, p. 433. ISBN 978-9975-82-223-7 (PDF).. |
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Cercetarea în biomedicină și sănătate: calitate, excelență și performanță 2021 | ||||||
Conferința "Cercetarea în biomedicină și sănătate: calitate, excelență și performanță" 1, Chişinău, Moldova, 20-22 octombrie 2021 | ||||||
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Background. In the local flora there are 3 species with different degrees of vulnerability from g. Galanthus. In order to restore the natural populations, the species were multiplied in vitro in the Plant Biotechnologies Laboratory of the “Alexandru Ciubotaru” Botanical Garden, and, subsequently, collections of vitroplants were created. Objective of the study. Comparative quantitative and qualitative study of alkaloids in different vegetable products in 3 species: Galanthus elweisii, G. nivalis and G. plicatus. Materials and methods. As biological material for the chemical analysis of alkaloids served: bulbs and aerial parts (leaves and flowers) from sp. G. elwesii, G. nivalis and G. plicatus, harvested in the flowering phase. The alkaloids were identified by colour and sedimentation reactions of the extracts, and their dosing by indirect titrimetric method. Results. Analytical reactions with Wagner Bouchardat and Dragendoff reagents, tannin, phosphowulframic, phosphomolybdenic, picric and picrolonic acids denote the presence of alkaloids. The dosage of the total alkaloids by the titrimetric method, in the recalculation to galantamine shows that the alkaloids content in the analyzed extracts varies from 0.238 to 0.674% depending on the species and plant product. For all species the alkaloid content (%) was higher in the aerial parts than in the bulbs: respectively, G.nivalis (0.590 and 0.351); G.elwesii (0.674 and 0.336) and G. plicatus (0.388 and 0.261). The values of the alkaloid content in G.nivalis and G.elwesii were almost equal. Conclusion. The obtained results indicate that the plants of all analyzed sp. G.elwesii, G.nivalis and G. plicatus multiplied in vitro and grown ex situ have the potential to accumulate alkaloids. In all species the vegetable product of aerial parts is richer in alkaloids than form bulbs. |
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Cuvinte-cheie G. elwesii, G. nivalis, G. plicatus, alkaloids, identification, dosing, G. elwesii, G. nivalis, G. plicatus, alcaloizi, identificare, dozare |
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