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SM ISO690:2012 PAVLOVSCHI, Elena, VEREGA, Grigore, STOIAN, Alina, NACU, Viorel. Surgery protocol of vascularized bone allotranslant. The next stage of in vivo experimental study. In: Cercetarea în biomedicină și sănătate: calitate, excelență și performanță, Ed. 1, 20-22 octombrie 2021, Chişinău. Chișinău, Republica Moldova: 2021, p. 333. ISBN 978-9975-82-223-7 (PDF).. |
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Cercetarea în biomedicină și sănătate: calitate, excelență și performanță 2021 | |||||
Conferința "Cercetarea în biomedicină și sănătate: calitate, excelență și performanță" 1, Chişinău, Moldova, 20-22 octombrie 2021 | |||||
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Background. Massive bone defects require a multidisciplinary therapeutic tactic. Bone transplantation is a successful approach, but insufficient in this case. Decellularized vascular bone allograft included in the host circuit may be the solution. Objective of the study. To protocol the in vivo intraoperative phase of the allografting of the vascularized femoral segment with the internal iliac artery in the domestic rabbit. Material and Methods. The 5 New Zealand White Rabbit were used, weighing 2.6-3.0 kg. We have studied the lateral intermuscular approach for the microsurgical grafting of the femur segment with the vascular pedicle (internal iliac artery). Being located between the upper part of the great trochanter and the distal 1/3 of the femoral shaft. The surgical technique was staged and recorded respecting vascular continuity. Results. We documented the lateral intermuscular surgical approach of the thigh, for grafting the femoral segment. The procedure was divided into 4 stages: 1. Preparation of the operating field 2. Incision of skin tissue, superficial fascia, and fascia lata. Next was preparation of access between m. vastus lateralis and short head of m. biceps femoris, with access to the lateral part of the diaphysis of femur, located between m. crureus and m. vast intermedius 3. Performing segmental osteotomy (2.5 cm long) of the femur with the preparation of the vascular pedicle to the internal iliac artery, with the formation of massive bone defect 4. Layer suturing of the surgical wound. Conclusion. The lateral approach of the thigh is a convenient one in the procedure of vascularized bone transplantation of the femur to a laboratory animal-New Zealand White Rabbit domestic rabbit. |
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Cuvinte-cheie vascularized bone allograft, protocol, invivo, allogrefă osoasă vascularizată, protocol, tehnică operatorie, in vivo |
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