Novooleksandrivskaya draft breed of horses was bred in Ukraine on the basis of Novooleksandrivskyay factory type horses of Russian draft breed of horses. Novooleksandrivskaya draft breed of horses, officially approved in 1998 , is currently represented in Ukraine by very limited livestock. When working to create a new breed, much attention was paid to creating a developed genealogical structure, which includes both male lines and mare’s families. Novooleksandrivskaya draft breed of horses belongs to small draft breeds. But in comparison with the original Russian draft breed, modern horses of the Novooleksandrivskaya draft breed of horses are larger, which is due to the use in working with the breed of crossbreeds with the Soviet draft breed, Perseron, Russian and Oryol trotting and riding breeds. All, the influx of blood from other breeds were carried out through the uterus. Horses of the Novooleksandrivskaya draft breed are characterized by a harmonious physique, strong, dry constitution, dampness of joints is extremely rare, elongated, powerful body, strong limbs of the correct structure and posture. Great importance is attached to the correct structure of the hooves and the density of the hoof horn, as horses of the Novooleksandrivskaya draft breed must tolerate long-term grazing and intensive work in different regions of Ukraine, from the steppes of Kherson to the Carpathian Mountains. Goodwill and recoil in work - a distinctive feature of horses of the Novooleksandrivskaya draft breed. They have high reproduction rates and are undemanding to the conditions of feeding and keeping. The use of such techniques as breeding in mares families in breeding work is just as important as breeding along male lines, since the animal receives half of the genotype from the mother. During the study, representatives of 13 old families mare's were identified, namely: BORONKI, (06) BRUSNIKI, VERBOVOI, ZAIMKI, ZEMLYNKI, (028) KOVARNOY, (1147) KOLYONOY, KRALI, (664) LUKAVOI, (327) NAIDI, (161) ROCKETS, (0919) TOGI, (0923) TUNGUSKI.In addition, identified representatives 12 new unregistered families and nests mare’s. Of these, 9 groups of mares, descended from a common ancestor, meet the requirements of families(47 HERALDIKI, (437) GRECHKI, (1848) KENGI, (1902) LAVES, 166 REPETICIYA, RAZNORYADKI, 176 RUDI, 236 TETIVI, FACTURI). 3 groups of mares, coming from a common ancestor, meet the requirements for nests (LOGUNI 95, 146 NEGI 04, MALUTKI). Also studied geological lines stallions sires. At present, only 3 lines of stallions remain in the breed, namely: (935) KOKETLIVOVO, (1390) TANTALA, (909) GRADUSA. There are no stallion lines left for 2021: (109) GAZONA , (200) CAPITENA , (1111) STYLYA, only the uteri of these lines remain. Currently, the best representatives of the breed are horses bred in the branch "Novooleksandrivsky equestrian plant № 64" SE "Horse breeding of Ukraine". To maintain a certain heterozygosity in a breed of horses with a limited gene pool, it is necessary to maintain a branched genealogical structure. It is necessary to continue work on selection and selection assessment of new genealogical branches and their registration as independent families and factory nests.