For the first time, to obtain blastodermal chimeras of ducks, 4-butandiol dimethanesulfonate (busulfan) was used as an agent that inhibits the development of primary germ cells, an alkylating agent whose mechanism of action is based on the cross-linking of DNA strands, as a result of which the replication process is disrupted. A method has been developed for creating germintic ducks chimeras using busulfan injections. It has been shown that duck embryos are more sensitive to busulfan than chick embryos. Injection of busulfan at a concentration of 300 ng per egg leads to 95.0-96.3% mortality of duck embryos. More than 50% of embryos died in the first 2-3 days after the start of incubation. Head and neck disorders were observed in 1.2% of embryos. When using a busulfan concentration of 150 ng per egg, a mortality rate of 33.3-75.3% was observed. A decrease in concentration to 75 ng led to 18.75-38.5% of embryonic mortality. In order to assess the egg productivity of daughters of drakes-chimeras created by injecting Shaoxin blastodermal cells (donors) into embryos of the Shaoxin breed (recipients), three groups of ducks of different origins (daughters of Shaoxin and Shaoxin drakes and hermentative chimeras) were analyzed. In total, 146 daughters of 12 Shaoxin drakes, 75 daughters of 11 Shaoxin drakes, and 157 daughters of 6 hermentative chimeras drakes were examined. The analysis of the productivity of daughters of germinative chimeras of ducks showed that, in general, chimerization of their parents did not affect the performance of daughters. An analysis of the productivity of a group of daughters obtained from chimeric animals indicates that, according to most indicators, this group occupies an intermediate position between the groups whose breeds served as donors and recipients. The method we have used to obtain chimeras can be successfully used on ducks in order to preserve genetic resources. Preservation of frozen germ cells of rare bird species and native bird breeds with the prospect of their reproduction using germinal chimeras will reduce the risks of a decrease in the genetic diversity of birds.